| 质粒名称: | pCMV-dR8.2 dvpr |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 8455 |
| 存储实验室: | Bob Weinberg |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | pHR' CMV 8.2 deltaR,pCMV-dR8.2 dvpr,pCMVdR8.2 dvpr |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV Forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-dR8.2 dvpr was a gift from Bob Weinberg (Addgene plasmid # 8455 ; http://n2t.net/addgene:8455 ; RRID:Addgene_8455) |
| 参考文献部分: | Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD. RNA 2003 Apr;9(4):493-501. 10.1261/rna.2192803 PubMed 12649500 |
买家导航
pCMV-dR8.2 dvpr载体质粒基本信息
pCMV-dR8.2 dvpr质粒图谱载体图谱和pCMV-dR8.2 dvpr载体序列质粒序列多克隆位点信息
pCMV-dR8.2 dvpr质粒载体简介
pCMV-dR8.2 dvpr质粒序列载体序列
LOCUS pCMV-dR8.2 dvpr 13380 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION 2nd generation lentiviral packaging plasmid. Can be used with 2nd
and 3rd generation transfer vectors. Use in conjunction with an
envelope plasmid such as pCMV-VSV-G..
ACCESSION .
VERSION .
KEYWORDS pCMV-dR8.2 dvpr
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13380)
AUTHORS Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS,
Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
TITLE Lentivirus-delivered stable gene silencing by RNAi in primary cells.
JOURNAL RNA 2003 Apr;9(4):493-501.
PUBMED 12649500
REFERENCE 2 (bases 1 to 13380)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13380
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(26..43)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(197..785)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(277..296)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(956..1816)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1579..1598
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1817..1921)
/gene="bla"
/label=AmpR promoter
primer_bind complement(2045..2067)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2167..2186
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2400..2419
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 2709..2727
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2709..2726
/label=SP6
/note="SP6 promoter, forward primer"
CDS complement(3964..3990)
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
misc_feature 4513..4746
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS complement(6043..9054)
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 6241..6358
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(8847..10349)
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
primer_bind complement(10599..10623)
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter complement(10620..10823)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(10649..10669)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 10824..11203
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
CDS complement(11653..11673)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 11803..11868
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
promoter complement(13002..13331)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 13016..13151
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(13070..13089)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 13316..13336
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
1 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
61 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
601 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
781 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
1021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
1081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
1141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
1201 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
1261 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
1321 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
1381 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
1441 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
1501 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
1561 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
1621 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
1681 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
1741 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
1801 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
1861 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
1921 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
1981 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
2041 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
2101 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
2161 actatgcggc atcagagcag attgtactga gagtgcacca ttcgacgctc tcccttatgc
2221 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
2281 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
2341 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
2401 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
2461 atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt
2521 tccgggtgcg ggacggcgtt accagaaact cagaaggttc gtccaaccaa accgactctg
2581 acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc tacacaatta
2641 ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
2701 cacatacgat ttaggtgaca ctatagaata cacggaatta attctagact aagaaaccat
2761 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga
2821 attctcatgt ttgacagctt atcatcgcca ctccagccag ccccactcat gttccccccg
2881 caccacatat gcacatgcac gcatgtacac acaacttgta gtgctctttc aaaggtttta
2941 ttcattgcag aggggtggac agggtagtgg tgggcctagt tgcagtagtt ctccagttgg
3001 tagagagagc agatgctggt gcagcactga tccacgatgc cgcgcttctg ccgggccacc
3061 tccagtgcca aggtctgaag gtcaccgggg cggccgctca gcagttcttg aagtactccg
3121 gatgcagctc tcgggccatg tgatgaaatg ctaggcggct gtcaaacctc cactctaata
3181 cttctctctc cgggtcatcc atcccatgca ggctcacagg gtgtaacaag cgggtgttct
3241 ctccttcatt ggcttcttct accttctctt gctcaactgg tactagcttg tagcaccatc
3301 caaaggtcag tggatatctg atccctggcc ctggtgtgta gttctgccaa tcagggaagt
3361 agccttgtgt gtggtagatc cacagatcaa ggatatcttg tcttcgttgg gagtgaatta
3421 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
3481 tcattggtct taaaggtacc tgaggtgtga ctggaaaacc cacctcctcc tcctcttgtg
3541 cttctagcca ggcacaatca gcattggtag ctgctgtatt gctacttgtg attgctccat
3601 gtttttctag gtctcgagat actgctccca ccccatctgc tgctggctca gctcgtctca
3661 ttctttccct tacagtaggc catccaatca cactactttt tgaccacttg ccacccatct
3721 tatagcaaaa tcctttccaa gccctgtctt attcttctag gtatgtggcg aatagctcta
3781 caagctcctt gtactacttc tataacccta tctgtcccct cagctactgc tatggctgtg
3841 gcattgagca agctaacagc actattcttt agctcctgac tccaatattg taggagattc
3901 caccaatatt tgagggcttc ccaccccctg cgtcccagaa gttccacaat cctcgttaca
3961 atcaagagta agtctctcaa gcggtggtag ctgaagaggc acaggctccg cagatcgtcc
4021 cagataagtg ccaaggatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
4081 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
4141 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
4201 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
4261 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
4321 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
4381 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
4441 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
4501 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
4561 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
4621 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
4681 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
4741 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
4801 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
4861 tccctcatat ctcctcctcc aggtctgaag atcagcggcc gctcaactac agatcatcaa
4921 tatcccaagg agcatggtgc cccatttcca cccccatctc cacaagtgct gatacttctc
4981 cttcactctc attgccactg tcttctgctc tttctattag tctatcaatt aacctgtcta
5041 tttttctttg tcttaatatt ttcctatatt ctatgattac tatggaccac acaactattg
5101 ctattattat tgctactact aatgctacta ttgctactat tataggttgc attacatgta
5161 ctacttactg ctttgataga gaagcttgat gagtctgact gttctgatga gctcttcgtc
5221 gctgtctccg cttcttcctg ccataggaga tgcctaaggc ttttgtcatg aaacaaactt
5281 ggcaatgaaa gcaacacttt ttacaatagc aattggtaca agcagtttta ggctgacttc
5341 ctggatgctt ccagggctct agtctaggat ctactggctc catttcttgc tctcctctgt
5401 cgagtaacgc ctattctgct atgtcgacac ccaattctga aatggataaa cagcagttgt
5461 tgcagaattc ttattatggc ttccacttcg gtccgccccg ggttcgggct actaaagctc
5521 tagtgtccat tcattgtatg gctccctctg tggcccttgg tcttctgggg cttgttccat
5581 ctgtcctctg tcagtttcct aacactaggc aaaggtggct ttatctgttt tggttttatt
5641 aatgctgcta gtgccaagta ctgtagagat cctaccttgt tatgtcctgc ttgatattca
5701 cacctaggac taactatacg tcctaatatg gtatttctta tagcagattc tgaaaaacaa
5761 tcaaaatagt gcagatgaat tagttggtct gctaggtcag ggtctacttg tgtgctatat
5821 ctctttttcc tccattctat ggagactccc tgacccaaat gccagtctct ttctcctgta
5881 tgcagacccc aatatgttgt tattactaat ttagcatccc ctagtgggat gtgtacttct
5941 gaacttattt ttggattagt actttcatag tgatgtctat aaaaccagtc cttagctttc
6001 cttgaaatat acatatggtg ttttactaat cttttccatg tgttaatcct catcctgtct
6061 acttgccaca caatcatcac ctgccatctg ttttccataa tccctgatga tctttgcttt
6121 tcttcttggc actactttta tgtcactatt atcttgtatt actactgccc cttcaccttt
6181 ccagaggagc tttgctggtc ctttccaaac tggatctctg ctgtccctgt aataaacccg
6241 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
6301 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
6361 gtggatgaat actgccattt gtactgctgt cttaagatgt tcagcctgat ctcttacctg
6421 tcctataatt ttctttaatt ctttattcat agattctatt actccttgac tttggggatt
6481 gtagggaatg ccaaattcct gcttgatccc cgcccaccaa caggcggcct taactgtagt
6541 actggtgaaa ttgctgccat tgtctgtatg tactgttttt actggccatc ttcctgctaa
6601 ttttaagagg aagtatgctg tttcttgccc tgtctctgct ggaattactt ctgcttctat
6661 atatccactg gctacatgaa ctgctaccaa gataactttt ccttctaaat gtgtacaatc
6721 tagctgccat attcctgggc tacagtctac ttgtccatgc atggcttccc cttttagctg
6781 acatttatca cagctggcta ctatttcttt tgctactaca ggtggtaggt taaaatcact
6841 agccattgct ctccaattac tgtgatattt ctcatgttct tcttgggcct tatctattcc
6901 atctaaaaat agtactttcc tgattccagc actgaccaat ttatctactt gttcatttcc
6961 tccaattcct ttgtgtgctg gtacccatgc caggtagact ttttcctttt ttattaactg
7021 ctctattatt tgactgacta actctgattc actcttatct ggttgtgctt gaatgattcc
7081 caatgcatat tgtgagtctg tcactatgtt tacttctaat cccgaatcct gcaaagctag
7141 atgaattgct tgtaactcag tcttctgatt tgttgtgtcc gttaggggga caactttttg
7201 tcttcctctg tcagttacat atcctgcttt tcctaattta gtttccctat tggctgcccc
7261 atctacatag aaagtttctg ctcctattat gggttctttc tctaactggt accataactt
7321 cactaaggga ggggtattga caaactccca ctcaggaatc caggtggctt gccaatactc
7381 tgtccaccat gcttcccatg tttccttttg tatgggtaat ttaaatttag gagtctttcc
7441 ccatattact atgctttctg tggctatttt ttgtactgcc tctgttaatt gtttcacatc
7501 attagtgtgg gcacccttca ttcttgcata ctttcctgtt ttcagatttt taaatggctc
7561 ttgataaatt tgatatgtcc attggccttg cccctgcttc tgtatttctg ctattaagtc
7621 ttttgatggg tcataataca ctccatgtac cggttctttt agaatctccc tgttttctgc
7681 cagttctagc tctgcttctt ctgttagtgg tactacttct gttagtgctt tggttcccct
7741 aagaagttta cataattgcc ttactttaat ccctgcataa atctgacttg cccaattcaa
7801 ttttcccact aatttctgta tgtcattgac agtccagctg tccttttctg gcagcactat
7861 aggctgtact gtccatttat caggatggag ttcataaccc atccaaagga atggaggttc
7921 tttctgatgt tttttgtctg gtgtggtaaa tccccacctc aacagatgtt gtctcagttc
7981 ctctattttt gttctatgct gccctatttc taagtcagat cctacataca aatcatccat
8041 gtattgatag atgactatgt ctggattttg ttttctaaaa ggctctaaga tttttgtcat
8101 gctacactgg aatattgctg gtgatccttt ccatccctgt ggaagcacat tgtactgata
8161 tctaatccct ggtgtctcat tgtttatact aggtatggta aatgcagtat acttcctgaa
8221 gtctttatct aagggaactg aaaaatatgc atcgcccaca tccagtactg ttactgattt
8281 tttctgtttt aaccctgcag gatgtggtat tcctaattga acttcccaga aatcttgagt
8341 tctcttatta agttctctga aatctactaa ttttctccat ttagtactgt cttttttctt
8401 tatggcaaat actggagtat tgtatggatt ttcaggccca atttttgaaa tttttccttc
8461 cttttccatt tctgtacaaa tttctactaa tgcttttatt ttttcttctg tcaatggcca
8521 ttgtttaact tttgggccat ccattcctgg ctttaatttt actggtacag tctcaatagg
8581 actaatggga aaatttaaag tgcagccaat ctgagtcaac agatttcttc caattatgtt
8641 gacaggtgta ggtcctacta atactgtacc tatagcttta tgtccgcaga tttctatgag
8701 tatctgatca tactgtctta ctttgataaa acctccaatt ccccctatca tttttggttt
8761 ccatcttcct ggcaaattca tttcttctaa tactgtatca tctgctcctg tatctaatag
8821 agcttccttt aattgccccc ctatctttat tgtgacgagg ggtcgctgcc aaagagtgat
8881 ctgagggaag ctaaaggata cagttccttg tctatcggct cctgcttctg agagggagtt
8941 gttgtctctt ccccaaacct gaagctctct tctggtgggg ctgttggctc tggtctgctc
9001 tgaagaaaat tccctggcct tcccttgtgg gaaggccaga tcttccctaa aaaattagcc
9061 tgtctctcag tacaatcttt catttggtgt ccttcctttc cacatttcca acagcccttt
9121 ttcctagggg ccctgcaatt tttggctatg tgcccttctt tgccacaatt gaaacactta
9181 acagtctttc tttggttcct aaaattgcct ttctgtatca ttatggtagc tggatttgtt
9241 acttggctca ttgcttcagc caaaactctt gctttatggc cgggtccccc cactccctga
9301 catgctgtca tcatttcttc tagtgtcgct cctggtccca atgcttttaa aatagtctta
9361 caatctgggt tcgcattttg gaccaacaag gtttctgtca tccaattttt tacctcttgt
9421 gaagcttgct cggctcttag agttttatag aatcggtcta catagtctct aaagggttcc
9481 tttggtcctt gtcttatgtc cagaatgctg gtagggctat acattcttac tattttattt
9541 aatcccagga ttatccatct tttatagatt tctcctactg ggataggtgg attatgtgtc
9601 atccatccta tttgttcctg aagggtacta gtagttcctg ctatgtcact tccccttggt
9661 tctctcatct ggcctggtgc aataggccct gcatgcactg gatgcactct atcccattct
9721 gcagcttcct cattgatggt ctcttttaac atttgcatgg ctgcttgatg tccccccact
9781 gtgtttagca tggtgtttaa atcttgtggg gtggctcctt ctgataatgc tgaaaacatg
9841 ggtatcactt ctgggctgaa agccttctct tctactactt ttacccatgc atttaaagtt
9901 ctaggtgata tggcctgatg taccatttgc ccctggatgt tctgcactat agggtaattt
9961 tggctgacct gattgctgtg tcctgtgtca gctgctgctt gctgtgcttt tttcttactt
10021 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
10081 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
10141 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
10201 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
10261 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgatc taattctccc
10321 ccgcttaata ctgacgctct cgcacccatc tctcaccagt cgccgcccct cgcctcttgc
10381 cgtgcgcgtc aacgcggccg cggacctcga agcttattac gggatcgatc cgtcgcataa
10441 gaagccaagg gggtgggcct atagactcta taggcggtac ttacgtcact cttggcacgg
10501 ggaatccgcg ttccaatgca ccgttcccgg ccgcggaggc tggatcggtc ccggtgtctt
10561 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
10621 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
10681 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
10741 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
10801 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
10861 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
10921 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
10981 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
11041 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
11101 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
11161 cccgtaattg attactatta ataactagtc aataatcaat gtcgggcgag ctcgaattcc
11221 tgcagcccaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
11281 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt
11341 caaggcacta tacatcaaat attccttatt aaccccttta caaattaaaa agctaaaggt
11401 acacaatttt tgagcatagt tattaatagc agacactcta tgcctgtgtg gagtaagaaa
11461 aaacagtatg ttatgattat aactgttatg cctacttata aaggttacag aatatttttc
11521 cataattttc ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc
11581 aagagttcta ttactaaaca cagcatgact caaaaaactt agcaattctg aaggaaagtc
11641 cttggggtct tctacctttc tcttcttttt tggaggagta gaatgttgag agtcagcagt
11701 agcctcatca tcactagatg gcatttcttc tgagcaaaac aggttttcct cattaaaggc
11761 attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata cacaaacaat
11821 tagaatcagt agtttaacac attatacact taaaaatttt atatttacct tagagcttta
11881 aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa
11941 gatccggggc ccactcataa atccagttgc cgccacggta gccaatcacc gtatcgtata
12001 aatcatcgtc ggtacgttcg gcatcgctca tcacaatacg tgcctggacg tcgaggattt
12061 cgcgtgggtc aatgccgcgc cagatccaca tcagacggtt aatcatgcga taccagtgag
12121 ggatggtttt accatcaagg gccgactgca caggcggttg tgcgccgtga ttaaagcggc
12181 ggactagcgt cgaggtttca ggatgtttaa agcggggttt gaacagggtt tcgctcaggt
12241 ttgcctgtgt catggatgca gcctccagaa tacttactgg aaactattgt aacccgcctg
12301 aagttaaaaa gaacaacgcc cggcagtgcc aggcgttgaa aagattagcg accggagatt
12361 ggcgggacga atacgacgcc catatcccac ggctgttcaa tccaggtatc ttgcgggata
12421 tcaacaacat agtcatcaac cagcggacga ccagccggtt ttgcgaagat ggtgacaaag
12481 tgcgcttttg gatacatttc acgaatcgca accgcagtac caccggtatc caccaggtca
12541 tcaataacga tgaagccttc gccatcgcct tctgcgcgtt tcagcacttt aagctcgcgc
12601 tggttgtcgt gatcgtagct ggaaatacaa acggtatcga catgacgaat acccagttca
12661 cgcgccagta acgcacccgg tacagaccgc cacggcttac ggcaataatg cctttccatt
12721 gttcagaagg catcagtcgg cttgcgagtt tacgtgcatg gatctgcaac atgtcccagg
12781 tgacgatgta tttttcgctc atgtgaagtg tcccagcctg tttatctacg gcttaaaaag
12841 tgttcgaggg gaaaataggt tgcgcgagat tatagagatc tggcgcacta aaaaccagta
12901 tttcacatga gtccgcgtct ttttacgcac tgcctctccc tgacgcggga taaagtggta
12961 ttctcaaaca tatctcgcaa gcctgtcttg tgtccaagct ttttgcaaaa gcctaggcct
13021 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
13081 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
13141 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
13201 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
13261 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
13321 aactgacaca cattccacag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
//
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